Figure 7
From: Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
Detection of DENV-2 antigens in liver of infected mice. (a) Negative control of a non-infected mouse liver incubated with anti-DENV-2 and anti-human IgG–horseradish peroxidase conjugate (bar=2 μm). (b) Liver of a 7-day DENV-2-infected mouse incubated with only anti-human IgG conjugate (bar=5 μm). (c–e) Mouse livers obtained 2 days (c and d) or 7 days (e) p.i., incubated with anti-DENV-2 and anti-human IgG conjugate (bars=5, 2 and 3 μm, respectively). Note focal hepatocytes exhibiting virus antigens around portal space (c) and central vein (e) (white arrows). Virus antigens were also observed in hyperplasic Kupffer cells inside sinusoid capillaries (d) and endothelial cells (e) (black arrow). CV, central vein; PS, portal space; KC, Kupffer cells.
