Figure 7

(a) Representative gel images of RT-PCR results GAPDH and mouse HSP27 mRNAs extracted after treatment with vehicle (0.4% fatty acid free BSA) or 0.1 mg/kg sphinganine-1-phosphate i.v. before 5 h. Densitometric quantification of relative mRNA band intensities normalized to GAPDH from RT-PCR reactions are shown in the bottom panel (N=6). Data are presented as means±s.e.m. ^*P<0.05 vs vehicle group. (b) Representative immunoblots for phospho (p)- and total ERK, phospho (p)- and total Akt, phospho (p)- and total HSP27 from renal cortices and liver of mice (right panel) after injection with vehicle or 0.1 mg/kg sphinganine-1-phosphate i.v. before 15 min. (c) Representative immunoblots for total HSP27 and actin (showing equal lane loading) from renal cortices and liver of mice after injection with vehicle or 0.1 mg/kg sphinganine-1-phosphate i.v. before 24 h. Densitometric quantifications of relative band intensities are also shown (left panel, N=4). ^*P<0.05 vs vehicle. Error bars, 1 s.e.m.