Figure 9

Hepatic expression of Acyl-CoA oxidase (ACO) (a) carnitine palmitoyltransferase 1-a (CPT-1) (b), uncoupling protein-2 (UCP-2) (c), or PPARα (d), mRNA in wt or Gclm null (ko) mice after 21 days on either a methionine-choline-sufficient (MCS), or methionine-choline-deficient (MCD) diet. ^ΦP<0.05 compared with wt MCD; ^*P<0.05 compared with wt MCS; ^#P<0.05 compared with ko MCS. Relative expression was determined by the ΔΔCt method as described in the Materials and Methods section, in which hepatic expression in wt, chow-fed, age-matched, male mice is 1.0.