Figure 1
From: ErbB4 promotes cyclooxygenase-2 expression and cell survival in colon epithelial cells
ErbB4 stimulates COX-2 expression in colon epithelial cells. (a) YAMC cells were infected with retroviral vectors expressing each ErbB4 isoform (A1, JM-a/CYT-1; A2, JM-a/CYT-2; B1, JM-b/CYT-1; B2, JM-b/CYT-2). Whole-cell lysates were prepared and subjected to western blot analysis for full-length (FL) ErbB4 and COX-2 expression. Actin blot included as loading control. (b) JM-b/CYT-2-expressing cells (YAMC-ErbB4) were exposed to HRG (100 ng/ml) for 3 h; COX-2 protein levels were determined by immunoblot analysis. (c) MDCK cells were infected with vector or ErbB4-expressing constructs and COX-2 levels were determined by western blot analysis.
