Figure 4
From: ErbB4 promotes cyclooxygenase-2 expression and cell survival in colon epithelial cells
EGFR regulates ErbB4 activation and COX-2 expression. (a) YAMC-ErbB4 cells were incubated with the EGFR inhibitor AG1478 (150 nM) for 30 min before 3 h treatment with TNF or HRG. COX-2 levels were determined by western blot analysis. (b) YAMC-ErbB4 cells were transfected with nontargeting or EGFR-specific siRNA pools for 72 h, then stimulated with TNF or HRG. EGFR, ErbB4 and COX-2 levels were determined by immunoblot analysis. (c) YAMC-ErbB4 cells were transfected with nontargeting or EGFR-specific siRNA pools, then stimulated with HRG for 15 min. ErbB4 phosphorylation was determined by western blot analysis. (d) YAMC-Vec and YAMC-ErbB4 cells were exposed to HRG for indicated times; EGFR and ErbB4 phosphorylation were determined by western blot analysis.
