Figure 2 | Laboratory Investigation

Figure 2

From: Fibroblasts stimulated via HLA-II molecules produce prostaglandin E2 and regulate cytokine production from helper T cells

Figure 2

Cytokine production of activated Th cells following MLR culture with allogeneic DCs in the presence of soluble factors produced by HLA-II-stimulated HGFs. Naïve Th cells were cultured with allogeneic DCs (T cells/DC ratio 10:1) for 7 days. After the cellular expansion by MLR culture, Th cells were collected and re-stimulated with anti-CD3/CD28 mAbs for 2 days, and culture supernatants were harvested to determine IFN-γ, IL-5, and IL-13 concentrations by ELISA. The ratios of IL-13/IFN-γ and IL-5/IFN-γ were calculated and were indicated by the mean value±s.d. of triplicate cultures of one set of T-cell-DC-HGF. Soluble factors produced from HGF stimulated with HLA-II mAbs, including anti-DR (L243; shaded columns) and anti-DQ mAbs (1a3; closed column), or with isozyme-matched control antibodies (open columns) were diluted (final concentration: 1/2, 1/4, 1/8) and added into the MLR culture between DCs and Th cells (a) or the culture of DCs to induce maturation (b). Representative data of six independent experiments are shown (*P<0.05).

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