Figure 1 | Laboratory Investigation

Figure 1

From: Tumor-initiating activity and tumor morphology of HNSCC is modulated by interactions between clonal variants within the tumor

Figure 1

Determining whether cell loss or coinjection with fibroblasts or keratinocytes alters tumor-initiating activity and tumor cell viability. (a) Detroit 562 cells stably expressing GFP were implanted subcutaneously in NOD/SCID mice and fluorescence was monitored over time (days). The site of injection is indicated with the arrow. Spurious fluorescence outside the injection site is due to autofluorescence of any remaining fur in the surrounding area. (b) Fluorescent intensity was measured in mice after injection of 107 GFP-expressing Detroit 562 cells. Initial fluorescence was arbitrarily set at 100%. Data are presented as mean±range of two independent experiments. (c) Detroit 562 cells were injected alone or in combination with normal human dermal fibroblasts (HDFs) or normal human epidermal keratinocytes (HEKs). The total monitoring period was 90 days. Data are presented as the number of mice bearing tumors/total number of mice injected.

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