Figure 3
HNSCC clonal variants modulate each others’ tumor-initiating activity. An admix study was performed using varying numbers of the Detroit 562 cells or the most (clone 1) and least (clone5) tumorigenic clonal variants of the Detroit 562 cell line. (a) Tumor-initiating activity presented as numbers of mice bearing tumors/total number of mice injected. The average time (days) to onset of tumors is also shown. (b) Hematoxylin and eosin-stained histological sections of the Detroit 562 parental cell line, clone1, clone 5 and an admix of clone 1 (103 cells)+clone 5 (3 × 104 cells). The different morphology of cells and the tumor organization in the parental cell line (arrowheads) vs clonal variants must be noted. The differences in the structure of the stromal elements within clone 5 and clone 1 (arrowheads) must also be noted. Finally, the relative basophilia of clone 5 tumor cells vs clone 1 cells and the presence of lacunae-like structures (asterisks) within clone 5 tumors must be noted.
