Figure 4
Expression of CEACAM6 or MMP9 in clonal variants in vitro and in vivo. Markers for clone 1 (CEACAM6) and clone 5 (MMP9) of the Detroit 562 parental cells were used to identify the different clones either in vitro or in vivo. (a) Detroit 562 parental cells or clone 1 or clone 5 variants were cultured on chamber slides and stained for CEACAM6 or MMP9 expression immunohistochemically ( × 40 magnification). A representative slide from at least three independent experiments is shown. (b) CEACAM6 and MMP9 expression was determined, immunohistochemically, in tumors derived from the Detroit 562 parental cell line, clone 1, clone 5, clone 1 (1000)+clone 5 (3 × 104) or in a patient oral SCC ( × 20 magnification). In some instances, focal expressions of CEACAM6 or MMP9 are highlighted by arrowheads. (c) Hematoxylin and eosin staining or CEACAM6 or MMP9 expression on serial sections of two regions of a tumor derived from clone 1+5 as described in panel b ( × 20 and × 40 magnification). Inset panels depict IgG-negative controls.
