Figure 10 | Laboratory Investigation

Figure 10

From: Glial dystrophin-associated proteins, laminin and agrin, are downregulated in the brain of mdx mouse

Figure 10

(a) Total RNA (1 μg) was reverse transcribed to cDNA and used in real-time PCR to evaluate the messenger expression, using specific primers for murine aquaporin-4, αβ-dystroglycan, Kir 4.1, α-syntrophin, laminin and agrin. The mdx mice brains have a higher expression in all the genes screened compared with the controls as shown in the histogram correspondent to their relative fold change. In this experiment, the fold change is normalized to actin. Data are representative of three independent experiments. (b) Western blotting analysis of aquaporin-4, αβ-dystroglycan, Kir 4.1, α-syntrophin, laminin and agrin in brain homogenates of mdx and control (ctr) mice. Quantification of the bands revealed a significantly higher level of all the protein in mdx brain with respect to the control. The data are representative of three independent experiments. (c) Western blotting analysis of short dystrophin isoform (Dp71) showing a lower expression in mdx brain with respect to the control. The absence of signal corresponding to 427-kDa molecular weight indicated the specificity of antibody against the 71-kDa dystrophin isoform. The data are representative of three independent experiments.

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