Table 1 Primary antibodies used for immunogold electron microscopy
From: Glial dystrophin-associated proteins, laminin and agrin, are downregulated in the brain of mdx mouse
Protein | Catalog number | Species | Dilution immunofluorescence | Dilution immunohistochemistry | Source |
|---|---|---|---|---|---|
α DG | sc-53986 | Mouse | 1:20 | Chemicon Int. | |
β DG | NCL-b-DG | Mouse | 1:500 | Novocastra | |
Agrin | AGR-530 | Mouse | 1:5 | 1:5 | Stressgen Bio. |
Laminin | 2233PLA | Rabbit | 1:25 | 1:25 | Euro-Diagnostica |
AQP4 | sc-20812 | Rabbit | 1:50 | 1:25 | Santa Cruz Bio. |
Kir 4.1 | sc-23637 | Goat | 1:20 | 1:100 | Santa Cruz Bio. |
Dp71 (pan-specific antibody) | MANDRA1 | Mouse | 1:20 | Sigma | |
α-Syntrophin | sc-13757 | Goat | 1:20 | Santa Cruz Bio. |