Table 1 Primers and TaqMan probes used in quantitative real-time PCR for the measurement of glutamic oxaloacetic transaminase 1, glutamic oxaloacetic transaminase 2, superoxide dismutase 1, superoxide dismutase 2, glutathione peroxidase 1 and β-actin cDNAs in rat
cDNA | Oligonucleotide | Sequence | Product size (bp) |
|---|---|---|---|
Glutamic oxaloacetic transaminase 1 | Forward primer | 5′-CCAGGGAGCTCGGATCGT-3′ | 79 |
Reverse primer | 5′-GCCATTGTCTTCACGTTTCCTT-3′ | ||
TaqMan probe | 5′-CCACCACCCTCTCCAACCCTGA-3′ | ||
Glutamic oxaloacetic transaminase 2 | Forward primer | 5′-GAGGGTCGGAGCCAGCTT-3′ | 82 |
Reverse primer | 5′-GTTTCCCCAGGATGGTTTGG-3′ | ||
TaqMan probe | 5′-TTTAAGTTCAGCCGAGATGTCTTTC-3′ | ||
Superoxide dismutase 1 | Forward primer | 5′-AAAGGACGGTGTGGCCAAT-3′ | 121 |
Reverse primer | 5′-TCCACCTTTGCCCAAGTCAT-3′ | ||
TaqMan probe | 5′-CTCAGGAGAGCATTCCATCATTGGCC-3′ | ||
Superoxide dismutase 2 | Forward primer | 5′-CGTCACCGAGGAGAAGTACCA-3′ | 131 |
Reverse primer | 5′-GGCTCAGGTTTGTCCAGAAAAT-3′ | ||
TaqMan probe | 5′-AGGCGCTGGCCAAGGGAGAT-3′ | ||
Glutathione peroxidase 1 | Forward primer | 5′-CGTGCAATCAGTTCGGACAT-3′ | 131 |
Reverse primer | 5′-CTCACCATTCACCTCGCACTT-3′ | ||
TaqMan probe | 5′-AATGAAGAGATTCTGAATTCCCTCAAGT-3′ | ||
β-Actin | Forward primer | 5′-ACCCGCGAGTACAACCTTCTT-3′ | 80 |
Reverse primer | 5′-TATCGTCATCCATGGCGAACT-3′ | ||
TaqMan probe | 5′-CCTCCGTCGCCGGTCCACAC-3′ |
