Figure 3 | Laboratory Investigation

Figure 3

From: Effects of calcium oxalate monohydrate crystals on expression and function of tight junction of renal tubular epithelial cells

Figure 3

COM crystals induced disruption of tight junction, redistribution and dissociation of occludin and ZO-1. The polarized MDCK cells were incubated with 100 μg/ml COM crystals for 48 h and then processed for double immunofluorescence staining for occludin (in red) and ZO-1 (in green). In merged view (right column), colocalization of occludin and ZO-1 was observed in yellow. The polarized MDCK cells grown in COM-free medium (to which 100 μg/ml COM crystals were added and incubated for 30 min, but were then removed) served as control cells. Original magnification was × 200 for all panels.

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