Figure 1

Tubulointerstitial damage and peritubular cell loss in aging kidney. (a, b) Masson-trichrome staining showing renal cortical morphology in 4-month-old (4M; a) and 24-month-old (24M; b) rats. Bar=100 μm. (c) Interstitial injury score. n=10 for each group. Values are expressed as mean±s.e.m. ^*P<0.05 versus 4 M. (d, e) Staining for rat endothelial cell antigen (RECA)-1 in cortical peritubular cells in 4 M (d) and 24 M (e) rats. Bar=100 μm. (f) Percent volume of peritubular cells in renal cortex. n=10 for each group. Values are shown as mean±s.e.m. ^*P<0.05 versus 4 M. (g, h) Masson-trichrome staining showing glomerular morphology in 4-month-old (4M; g) and 24-month-old (24M; h) rats. Bar=50 μm. (i) Glomerular injury score. n=10 for each group. Values are expressed as mean±s.e.m. ^*P<0.05 versus 4 M.