Figure 2
From: Mitochondrial damage-induced impairment of angiogenesis in the aging rat kidney
Interstitial hypoxia and expression of vascular endothelial growth factor (VEGF) in aging rat kidney. (a, b) Renal cortical hypoxia detected with pimonidazole staining in 4-month-old (4M; a) and 24-month-old (24M; b) rats. Dashed line=corticomedullary border. Bar=0.5 mm. (c) Percent volume of pimonidazole-positive area in renal cortex. (d, e) Immunohistochemical staining for VEGF in the renal cortex in 4 M (d) and 24 M (e) rats. Bar=100 μm. (f) Percent volume of VEGF-positive area in renal cortex. n=10 for each group. Values are shown as mean±s.e.m. *P<0.05 versus 4 M.
