Figure 3
From: Mitochondrial damage-induced impairment of angiogenesis in the aging rat kidney
Accumulation of oxidative stress in aging rat kidney. (a, b) Malondialdehyde (MDA) staining in 4-month-old (4M; a) and 24-month-old (24M; b) rat kidneys. Bar=100 μm. (c) Percent volume of MDA-positive area in renal cortex. (d, e) Nitrotyrosine staining in 4 M (d) and 24 M (e) rat kidneys. Bar=100 μm. (f) Percent volume of nitrotyrosine-positive area in renal cortex. (g, h) 8-Hydroxy-2′-deoxyguanosine (8-OHdG) staining in 4 M (g) and 24 M (h) rat kidneys. Bar=100 μm. (i) Percent volume of 8-OHdG–positive area in renal cortex. n=10 for each group. (i) Western blot analysis for nitrotyrosine. Values are expressed as mean±s.e.m. *P<0.05 versus 4 M.
