Figure 6
From: Mitochondrial damage-induced impairment of angiogenesis in the aging rat kidney
Chromatin immunoprecipitation (ChIP) analysis of vascular endothelial growth factor (VEGF) promoter after hypoxia for 4 h. (a) Schematic for primers. Specific primer sets of VEGF promoter region containing two hypoxia-inducible factor (HIF)-1α binding sites (−1140 to −788) were used. (b) Hypoxia-induced HIF-1α activation. A ChIP (IP) assay was performed using an antibody specific for HIF-1α. Rabbit normal IgG was used as a negative control. n=6 for each group. Data are shown as mean±s.e.m. ND=not detected. *P<0.05 versus 21% O2. †P<0.05 versus 5% O2. (c) Mitochondrial damage decreased HIF-1α activation in response to hypoxia. Normoxia=21% O2; normal=normal tubular cell; +Myx=myxothiazol-treated normal proximal tubular cell; ρ0=ρ0 tubular cell. n=6 for each group. Data are expressed as mean±s.e.m. *P<0.05 versus normal cell at 2% O2.
