Figure 1 | Laboratory Investigation

Figure 1

From: Yersinia enterocolitica induces epithelial barrier dysfunction through regional tight junction changes in colonic HT-29/B6 cell monolayers

Figure 1

Characterization of barrier impairment. (a) HT-29/B6 monolayers were apically infected with 107 (multiplicity of infection (MOI) 10) or 108 Y. enterocolitica O9 (MOI 100) and incubated at 37°C. 2.5 h p.i. bacteria were killed by the addition of 100 μg/ml gentamycin. Rt was monitored over 48 h and was dose and time dependent (n=6, *P<0.05, **P<0.01 vs control). (b) No changes in ISC were obtained (n=18). (c) Permeability of infected and control monolayers was determined by flux measurement in modified Ussing-type chambers. Y. enterocolitica increased permeability for 182 Da mannitol (n=6, ***P<0.001 vs control) and of 332 Da fluorescein (n=6, **P<0.01 vs control). For 4000 Da fluorescein isothiocyanate-Dextran layers from both conditions were almost impermeable.

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