Figure 3
Paracellular leakiness and impaired tight junctions. (a) Repi, Rtrans and Rpara were measured by two-path impedance spectroscopy. Repi, reflecting the total epithelial resistance, as well as the paracellular resistance Rpara were reduced in Y. enterocolitica-infected HT-29/B6 monolayers. Data are presented as means±s.e.m. of n=6 (**P<0.01, ***P<0.001 vs control). (b) Western blot analyses revealed reduced protein levels of claudin-2 (n=9, **P<0.01), claudin-3 (n=9, *P<0.05), claudin-8 (n=12, **P<0.01) and claudin-10 (n=6, **P<0.01), as well as of ZO-1 (n=6, **P<0.01) in infected HT-29/B6 monolayers compared with control. (c) Densitometric quantification of TJ proteins after normalization with β-actin (n=6–12).
