Figure 4 | Laboratory Investigation

Figure 4

From: Yersinia enterocolitica induces epithelial barrier dysfunction through regional tight junction changes in colonic HT-29/B6 cell monolayers

Figure 4Figure 4

Analyzes of tight junction proteins in leaky and intact regions by confocal laser scanning microscopy. The z-stack projections of infected monolayers displaying parallel biotinylation (red) and immunostaining for (a) ZO-1, (b) claudin-4 and (c) claudin-8 (green). ‘Leaky regions’ are characterized by an intense biotin permeation (red), which was absent in ‘intact regions’ that are characterized by lacking biotinylated cell boundaries.

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