Figure 7
Y. enterocolitica-induced cytotoxicity. (a) Induction of necrosis in HT-29/B6 monolayers was determined by measurement of LDH release. Furthermore, LDH release from controls and infected monolayers were shown in correlation to the corresponding Rt values at 48 h p.i. (n=12). (b) Induction of apoptosis was monitored by TUNEL-staining and subsequent fluorescence-activated cell sorting (n=5). HT-29/B6 monolayers treated with a combination of tumor necrosis factor α and interleukin-13 served as positive controls.
