Figure 2

TF expression in WT and DKO mouse eyes. (a, b) IHC demonstrated more intense TF staining (asterisks) in DKO retina compared with WT retina. (c) TF mRNA levels were analyzed and compared with that in young WT. Retinal lesions of DKO mice express higher TF mRNA levels than is observed in the normal retinal area of age-matched WT mice. Two sets of mice were analyzed and the retinal tissue from the second set of DKO mice (with more lesions) exhibited a higher TF expression level than was observed with the first set of animals. (d) TF mRNA expression in neuroretinal tissue increased in DKO mice when compared with age-matched WT mice. TF expression increased with age in WT and DKO mouse retina (^*P<0.05; ^**P<0.01; n=3). (e) TF mRNA expression in RPE cells isolated from the mice also increased with age. DKO RPE cells demonstrated significantly higher TF transcript level compared with age-matched WT cells (^*P<0.05; n=3). (f, g) TF antigen expression was elevated in cultured RPE cells isolated from WT mice compared with RPE cells isolated from DKO mice.