Figure 5 | Laboratory Investigation

Figure 5

From: Uropathogenic E. coli infection provokes epigenetic downregulation of CDKN2A (p16INK4A) in uroepithelial cells

Figure 5

UPEC infection of 5637 cells results in downregulation of CDKN2A and MGMT. Infection of 5637 cells with UPEC resulted in 3.3-fold decrease of CDKN2A mRNA (a) and a 9.1-fold decrease of MGMT mRNA expression (b) compared with non-inoculated cells. CDKN2A, MGMT and GAPDH expression was analyzed by real-time PCR. Quantitative analysis was performed by normalizing the CDKN2A or MGMT values to the corresponding GAPDH values and then calculating the ratio inoculated cells/non-inoculated (control) cells. Values represent mean of N=3±s.e.m. Student's t-test was used to calculate P-values.

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