Figure 4
From: The role of CD18 in the production and release of neutrophils from the bone marrow
The number of Gr-1+ cells grown from LSK cells in stroma-free culture. Isolated pure LSK cells were cultured for 14 days in serum-containing medium supplemented with IL-3, IL-6, SCF, G-CSF, and GM-CSF without other matrix or cells. Cells were obtained from three replicate wells of each genotype and stained with Annexin V and anti-Gr-1 as described in Materials and Methods section. The number of cells in each well was determined using a hemocytometer. Data are expressed as mean±s.e.m. of three replicates. *P<0.05, independent t-test.
