Figure 3 | Laboratory Investigation

Figure 3

From: Relationship of strain-dependent susceptibility to experimentally induced acute pancreatitis with regulation of Prss1 and Spink3 expression

Figure 3

Northern and western blots analyses on Spink3, Prss1, and amylase expressions in cerulein-induced acute pancreatitis. (a) Northern blot analysis; 18S and 28S were used as loading controls because G3PDH expression was induced by cerulein treatment. The same JF1 samples were arranged in the first four lanes of northern blots. (b) Densitometric analysis for Spink3 and Prss1 expressions without cerulein induction in northern blot. (c) Densitometric analysis for Spink3 and Prss1 expressions with cerulein induction in northern blot. (d) Western blot analysis. β-actin was used as a loading control. The same JF1 samples were arranged in the first four lanes of western blots. (e) Densitometric analysis for Spink3 and Prss1 expressions after cerulein induction in western blot. (f) Densitometric analysis for Spink3 and Prss1 expressions with cerulein induction in western blot. Unfilled bars represent untreated strains of mice, whereas black bars indicate the treated strains. ‘−’: saline treatment. ‘+’: cerulein treatment. *P<0.05. NS: no significance.

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