Figure 2 | Laboratory Investigation

Figure 2

From: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays

Figure 2

Summary of the changes in gene expression in untransformed MDCK cells grown in 2D and 3D environments to confluency or cell clusters. The mRNA was harvested from the specimens, converted to cDNA and used to probe the Affymetrix Canine arrays as described in MATERIALS AND METHODS. The shift of untransformed MDCK cells to a more complex 3D matrix induced a change of 6474 gene expression levels (Figure 2, Supplementary Table 3). However, after removing from the files the genes also altered by the v-Src, only 118 genes could be linked to a shift from 2D to 3D environment: very minor changes had occurred in the expression levels of cadherins or other junctional complexes and the most striking feature was a change in the expression of rab proteins. Also the expression of a rho/racGEF and GDI decreased. Especially interesting was a decrease in the expression level of an inhibitor of apoptosis (IAP) survivin.

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