Figure 3 | Laboratory Investigation

Figure 3

From: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays

Figure 3

Changes in mRNA expression levels of selected hits, Rab5, Rab7, Rab8 and survivin as determined by real-time PCR to validate the microarray results. The RT-PCR data were normalized to a control mRNA, ubiquitin-specific peptidase and the values were converted to fold-changes using the expression level in untransformed MDCK cells at 2D as a reference level. The appropriate primers are shown in the lower part of the figure.

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