Table 1 Co-localization of internalized surface cadherin and β-catenin in v-Src-transformed MDCK cells

From: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays

 

Co-localization coefficient 1 =Proportion of co-localized β-catenin from the total β-catenin area

Co-localization coefficient 2 =Proportion of co-localized surface E-cadherin from the total cadherin area

Co-localization coefficient 1 =Proportion of co-localized β-catenin from the total β-catenin area

Co-localization coefficient 2 =Proportion of co-localized surface E-cadherin from the total cadherin area

 

30 min at +40.5°C

30 min at +40.5°C

60 min at +40.5°C

60 min at +40.5°C

Apical

0.31

0.30

0.18

0.15

Basal

0.38

0.52

0.31

0.40

 

30 min at +35°C

30 min at +35°C

60 min at +35°C

60 min at +35°C

Apical

0.54

0.42

0.35

0.20

Basal

0.57

0.60

0.29

0.31

  1. Co-localization analysis of internalized antibody-labelled surface cadherin with β-catenin in v-Src-transformed MDCK cells 30 or 60 min after warming to +40.5°C or +35°C. Quantitative analysis was carried out using Olympus Fluoview1000 software after thresholding to eliminate noise and background from the images. According to Handbook of Confocal Microscopy49 the use of co-localization coefficients are the most biologically useful, representing the proportion of pixels from each channel that contribute to the co-localized area. Co-localization coefficients are higher at the 30 min time point, especially at +35°C, whereas in 60 min at +40.5°C apical cadherin vesicles are practically void of β-catenin.
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