Figure 1

Comparison of CD24 mAbs on carcinoma tissues. (a) Immunohistochemical staining of breast carcinoma sections using mAbs SN3b and SWA-11. Representative cases from a tissue microarray comprising 183 breast carcinomas are depicted to illustrate the different types of immunoreactivity obtained with both antibodies. The first panel (−/−) shows a case that is negative for both mAbs. The minor brownish shade seen for SWA11 does not justify to count this case as SWA11 positive, given the rather intense immunoreactivity else noted with this mAb. The second panel (−/+) shows completely SN3b-negative cases with strong SWA11 immunoreactivity. The third panel (+/−) shows the reverse: strong membranous and cytoplasmic SN3b staining in the absence of a significant SWA11 immunoreactivity. Lastly, in panel 4 a case strongly positive for both mAbs is shown. (b) Immunohistochemical staining of a renal carcinoma TMA (105 cases) with mAbs SN3b and SWA-11. Here, the overall weaker SN3b immunoreactivity is readily observable. Panel 1 (−/−) shows negative for both antibodies. Panel 2 (–/+) shows a moderate to strong SWA11 staining. Panel 3 (+/−) shows a weak SN3b staining, SWA11 is negative. Panel 4 (+/+) shows a weak SN3b staining, whereas SWA11 immunoreactivity is of moderate intensity. (c) Immunohistochemical staining of a prostate adenocarcinoma TMA (95 cases) with mAbs SN3b and SWA-11. In panel 1 (−/−) both cases are negative and in panel 2 (−/+) SN3b is negative, but SWA11 is weakly positive. Panel 3 (+/−) is weakly positive for SN3b but lacks SWA11 staining, and panel 4 (+/+) shows a moderate to strong staining with both mAbs. Magnification of all photographs is × 400.