Figure 2 | Laboratory Investigation

Figure 2

From: Molecular and clinical dissection of CD24 antibody specificity by a comprehensive comparative analysis

Figure 2

Comparison of CD24 mAbs on cancer tissues and cell lines. (a) Western blot analysis of breast carcinoma lysates. Tumor lysates (20 μg protein per lane) were analyzed with mAbs SN3b and SWA11 followed by peroxidase-conjugated goat anti-mouse IgG and IgM. < denotes a protein band only recognized by mAb SN3b. (b) FACS analysis of tumor cell lines. Cells were stained with the indicated mAbs (SWA-11, SN3b) to CD24 followed by PE-conjugated goat anti-mouse Ig secondary antibody (open curves). For background controls, the primary mAb was omitted (shaded curves). Note that SWA11 recognizes the protein core sequence of CD24. SN3b recognizes a carbohydrate epitope associated with CD24.

Back to article page