Figure 3 | Laboratory Investigation

Figure 3

From: Molecular and clinical dissection of CD24 antibody specificity by a comprehensive comparative analysis

Figure 3

The SN3b epitope can decorate the CD24 antigen. (a) Depletion of cell lysates (Nalm-2 or Nalm-6) with mAb SWA11-coupled sepharose codepletes SN3b reactivity. Please note that the lanes showing SWA11 precipitates are heavily overloaded because of bound antigen. (b) Affinity-purified CD24 from the indicated cell lines was analyzed by silver staining and western blot analysis as indicated above. (c) Purified CD24 from SKOV3ip or Nalm-2 cells was used for ELISA with mAbs SWA11 and SN3b followed by peroxidase-conjugated goat anti-mouse IgG and IgM. (d) Purified CD24 from SKOV3ip or Nalm-2 cells was used for ELISA with recombinant Siglec or selectin–Fc fusion proteins complexed with peroxidase-conjugated goat anti-human IgG.

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