Figure 4
The SN3b epitope is sialic acid dependent and is present in O-glycans. (a) Purified CD24 from Nalm-2 cells was digested with neuraminidases from A. urefaciens (Au), V. cholerae (Vc) and S. pneumoniae (Sp), PNGase F and Endo H. *Indicates the control. CD24 was analyzed by western blot using mAbs SN3b and SWA-11. (b) SKOV3ip cells were stably transfected with vectors encoding FUT3 (SKOV3/FUT3), FUT6 (SKOV3/FUT6) and mock transfected (SKOV3/mock) as indicated, and the expression of sLea, sLex and SN3b epitopes was analyzed by immunofluorescence microscopy. Antibodies used were anti-sLea CA19-9 (192) (red), anti-V5 (green), anti-sLex KM93 (red) and anti-SN3b (SN3b) (red). Staining of the nucleus was carried out with DAPI (blue).
