Figure 1 | Laboratory Investigation

Figure 1

From: Blockade of interleukin-6 signaling enhances hepatic steatosis but improves liver injury in methionine choline-deficient diet-fed mice

Figure 1

The effect of an MCD diet on plasma IL-6 and SAA levels and hepatic IL-6/ GP130-related gene expression. (a) Plasma IL-6 levels were measured. Mean±s.e. data from each group (n=5 per group) are plotted at 8 weeks. (b) Phosphorylated (p)-stat3, total (t)-STAT3, p-Akt, t-Akt, p-AMPK and t-AMPK levels were evaluated by immunoblot analysis of livers obtained from three mice per group after 8 weeks. To control for loading, the blot was stripped and reprobed for β-actin, a housekeeping gene. (c) Plasma SAA levels were measured. Mean±s.e. data from each group (n=5 per group) are plotted at 8 weeks (*P<0.05). (d) mRNA levels of SOCS3 were determined by quantitative real-time PCR analysis of total liver RNA obtained after 8 weeks. Results were normalized to glucuronidase (GUS) expression in each sample and then expressed as fold change relative to gene expression in chow-fed control mice. Mean±s.e. data from each group (n=5 per group) (P=0.094).

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