Figure 5

Neutrophil signaling pathways activated by flagellin. (a) Time course of p38, ERK, and Akt phosphorylation triggered by FliC. Neutrophils were incubated in the presence or absence of FliC 50 ng/ml for the indicated times at 37 °C. Kinase phosphorylation was then assessed by western blot. Immunoblots are representatives of at least three independent experiments. (b) Time course of IκBα degradation and p65 phosphorylation induced by flagellin. Neutrophils were incubated in the presence or absence of FliC 50 ng/ml for the indicated times at 37 °C. Then, IκBα degradation and Ser536-p65 phosphorylation were assessed by western blot. Immunoblots are representative of three independent experiments.