Figure 1
From: Overexpression of SERPIN B3 promotes epithelial proliferation and lung fibrosis in mice
(a–c) Homozygosis for human SERPIN B3 assessment by qualitative and quantitative PCR (a). Agarose gel electrophoresis for PCR of the housekeeping gene actin and SERPIN B3 DNA in emblematic cases. Actin is detected as a 425-base pair (bp) and SERPIN B3 as a 261-bp PCR product. Lane 1: DNA molecular weight marker; lane 2: SERPIN B3 TG animals; lane 3: WT mice; lane 4: negative control. Human SERPIN B3 mRNA expression in different organs of TG mice (b). The amount of transcripts was higher in the brain, lung and liver. Human and mouse SERPIN B3 mRNA expression in TG animals (c). Agarose gel electrophoresis for PCR of the mouse housekeeping gene actin, human and mouse SERPIN B3 in emblematic TG cases, both treated with BLM (+) and PBS (−). Actin is detected as 425-(bp), human SERPIN B3 as 261-bp and mouse SERPIN B3 as 228-bp PCR products. Human SERPIN B3 expression normalized to β-actin is displayed in the histogram.
