Figure 3
UVB-induced expression of CXCL1/KC in the skin of PLCɛ+/+ and PLCɛ−/− mice. (a) The shaved dorsal skins of PLCɛ+/+ (white bar) and PLCɛ−/− (black bar) mice were irradiated with a single dose of UVB at either 1 or 2.5 kJ/m2. The CXCL/1KC mRNA level in the skin collected at 0, 3, 6, 12, and 24 h was quantitated by qRT–PCR. The results are expressed as the fold change using the value of the non-irradiated skin as 1. **P <0.01. (b) Cultured keratinocytes and fibroblasts from were irradiated with a single dose of UVB at 0.1 kJ/m2 followed by culturing for another 24 h. Medium was removed before the UVB treatment. CXCL1/KC secretion in the supernatants was quantitated by ELISA and was calculated as pg/ml/105 cells/24 h. (c) After pre-treatment with 2.5 μM U73122 or 0.1% DMSO for 10 min, cultured keratinocytes and fibroblasts from PLCɛ+/+ mice were irradiated with a single dose of UVB at 1 kJ/m2 followed by culturing for another 24 h in the presence of either 2.5 μM U73122 or 0.1% DMSO. CXCL1/KC secretion in the supernatants was quantitated by ELISA and was calculated as pg/ml/105 cells/24 h. The results shown are representative of three independent experiments.
