Figure 4
Role of TNF-α in UVB-induced expression of CXCL1/KC in skin cells. (a) The shaved dorsal skins of PLCɛ+/+ (white bar) and PLCɛ−/− (black bar) mice were irradiated with a single dose of UVB at 1 kJ/m2. The TNF-α mRNA level in the skin collected at 0, 3, 6, 12, and 24 h was quantitated by qRT–PCR. The results are expressed as the fold change using the value of the non-irradiated skin as 1. (b) After pretreatment without or with 200 nM TAPI-2 for 1 h, cultured keratinocytes and fibroblasts from PLCɛ+/+ mice were irradiated with a single dose of UVB at 1 kJ/m2 followed by culturing for another 24 h in the absence or presence of 200 nM TAPI-2. CXCL1/KC secretion in the supernatants was quantitated by ELISA and was calculated as pg/ml/105 cells/24 h. The results shown are representative of three independent experiments.
