Figure 5

Suppression of the UVB-induced proliferative response in PLCɛ^−/− mice. (a) The epidermal thickness of PLCɛ^+/+ (white bar) and PLCɛ^−/− (black bar) mice at 0 and 168 h after irradiation with a single dose of UVB at 1 kJ/m² used in Figure 1 was calculated as described in materials and methods. Data are expressed as mean±s.d. ^*P<0.05. (b) The skin sections used in (a) were stained with the anti-PCNA antibody. Representative photographs of skin sections from PLCɛ^+/+ (+/+) and PLCɛ^−/− (−/−) mice at 168 h after irradiation are shown (upper two panels). Bar, 100 μm. The percentage of PCNA-positive cells in the basal cell layer against total basal cells in skin sections from PLCɛ^+/+ (+/+) and PLCɛ^−/− (−/−) mouse was determined as described in materials and methods and is expressed as mean±s.d. (lower panel). ^**P<0.01.