Figure 4 | Laboratory Investigation

Figure 4

From: The anti-TNF-α antibody infliximab indirectly regulates PECAM-1 gene expression in two models of in vitro blood cell activation

Figure 4

Western blot analysis of cell lysate from unstimulated, PHA- and LPS-stimulated U-937 cell line 4 h after treatment with antibodies specific for PECAM-1, ICAM-1 and β-actin, (Co=control) (a). Densitometry analysis of the western blots (b) was performed to show the changes in protein expression of PECAM-1 and ICAM-1. Results represent mean values±s.e.m., (*P<0.05, n=3). Western blot analysis of cell lysate from unstimulated, PHA- and LPS-stimulated human PBLs 4 h after treatment using antibody against PECAM-1 and ICAM-1 clearly showed a downregulation of PECAM-1 and an increase in ICAM-1 synthesis after treatment with PHA and LPS compared with unstimulated cells (n=3, c). Western blot analysis of cell lysate from human granulocytes after 2 h incubation with infliximab (IFX), TNF-α, IFN-γ, infliximab plus TNF-α compared with unstimulated cells (n=3, d).

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