Table 1 PCR and sequencing primers utilized for pyrosequencing assay

(a) PCR primers

Forward: 5′–GGGTTATTTGGTGATTAAGGTG–3′

Reverse: biotin-5′–AAAACCVTCCACCAAAAA–3′

(b) Sequencing primers

S1 Primer: 5′–TTTGGTGATTAAGGTGG–3′

Sequence to analyze

5′–TGGYGGTGGAYGGYGATTYGGGTTAGAAYGTTTGGTTGTYGTATTAG–3′

Dispensation order

5′–GTAGTCGTGTATCAGTCGTATCGTAGTATCGTGTAGTCGTA–3′

S2 Primer: 5′–YRGYRTGTGGGYGTATAAT–3′

Sequence to analyze

5′–GGYGAGGTGYGTATYGTTAGGTTGTTGAGYGAGYGYGAYG–3′

Dispensation order

5′–TGTCGAGTAGTCGTGATCGTAGTGTGATGTCGATGTCAGATCGTATC–3′

S3 Primer: 5′–TGGTGTTKGTTAAGGATAA–3′

Sequence to analyze

5′–TGGYGAGTTTTYGYGTTYGGTTATYGTTAYGTTGTAYGTGT–3′

Dispensation order

5′–GTAGTCGATGTTCAGTCTGTCGTGATCGTGATCGTGTGATCGTG–3′