Figure 3
Expression of invasion-related genes in SOX2-KD melanoma cell line. Seven genes studied in an array of invasion-related genes had a >80% decrease in expression in SOX2-KD cell line compared with control (a). Quantitative RT-PCR (b) and western blotting (c) of mRNA and protein expression of MMP-3 in SOX2-KD line compared with control. MMP-3 knockdown in the A2058 cell line was confirmed by quantitative RT-PCR (d) and western blotting (e). Cell invasion of MMP-3-KD cells was compared with control by in vitro invasion assay (f, g). Data in panels b, d, and f are presented as mean±s.d. *Marks significant (P<0.05) differences compared with the control group.
