Figure 4 | Laboratory Investigation

Figure 4

From: SOX2 contributes to melanoma cell invasion

Figure 4

SOX2 and MMP-3 immunoreactivity in patient and xenograft melanomas. In patients (a–f), SOX2 (brown) and MMP-3 (blue) immunopositivity is located in small nests (c, d) or single cells (e, f) infiltrating stroma, in contrast to tumors with non-invasive perimeters (a, b). Adjacent cores from tissue microarray (TMA) (g, h) show strong SOX2/MMP-3 co-expression in small nests infiltrating stroma (g) and weaker immunostaining in expansile tumorigenic zones without stroma (h). Xenograft melanomas demonstrate preferential co-expression of SOX2 (brown by IHC, red by IF) and MMP-3 (blue by IHC, green by IF) along tumor edge (i, k; dotted), with a decrease toward tumor center (j, l; nuclei=blue DAPI label). Xenografts from SOX2-KD cell line (m–p) were relatively devoid of SOX2 and MMP-3. (a–f, i–p) Bar=50 μm, (g, h) bar=500 μm.

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