Figure 2 | Laboratory Investigation

Figure 2

From: Sjögren's syndrome autoantibodies provoke changes in gene expression profiles of inflammatory cytokines triggering a pathway involving TACE/NF-κB

Figure 2

Anti-Ro/SSA autoantibodies (Abs) modulate nuclear factor-κB (NF-κB)-dependent transcriptional activity. (a) Confocal microscopy images from a representative experiment show anti-Ro/SSA Abs exposure increased NF-κB p65 nuclear immunofluorescence, as indicated in panel C. (b) Levels of NF-κB-motif-binding activity after 48 h of autoantibodies (Abs) stimulation. Nuclear extracts were isolated, and the level of endogenous NF-κB (p65) transcriptional activation was examined by using a TransAM NF-κB kit. Values shown are the means±s.e. from six independent experiments. ELISA-based assay shows NF-κB subunit p65–DNA binding was increased following anti-Ro/SSA Abs exposure.

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