Table 1 Primers and probes used in RT-PCR and real-time PCR assays
Sequences of the probes used in real-time PCR | |
|---|---|
Target gene | Probe (5′-FAM and 3′-TAMRA labeled) |
TACE | 5′-CTGTGCAGTAGGACACGCCT-3′ |
IL-1α | 5′-TCCACAGCGATTTATC-3′ |
IL-1β | 5′-GGATTCAATGAGGAGACTTGC-3′ |
IL-6 | 5′-CTGCCAAGAGAGCCACGGCCAG-3′ |
IL-8 | 5′-TGCCCAAGAAGGCCACAGAACTGAA-3′ |
IL-9 | 5′-TGCTGCCTCCAAGAACACAACTGA-3′ |
IL-10 | 5′-CTTCCCTGTGAAAACAAGAGCAAGGCC-3′ |
IL-13 | 5′-TTGCCCAAGCAGGCCACAGAATTG-3′ |
IL-17 | 5′-CCTGGCCAAGGTCATCCATGACAACTT-3′ |
IL-22 | 5′-TCAGCCAGTTCCAGCTCCTCATGCA-3′ |
TNF-α | 5′-TAGCCCATGTTGTAGCAAACCCTCAAGC-3′ |
IL-10RA | 5′-CGAGGTGACCTGGGCACCATCC-3′ |
IL-13RA | 5′-TAGCCCATGTTGTAGCAAACCCTCAA-3′ |
