Figure 3
CYP2E1 inhibition by DDTC. CYP2E1 (a) mRNA expression, (b) protein expression and (c) activity were measured 3 and 24 h after CCl4 treatment (1.5 ml/kg), with or without pretreatment with DDTC. Transcript levels of CYP2E1 were measured by RT-qPCR. Expression of S6 was used to normalize mRNA levels. CYP2E1 protein expression was assessed by western blotting and normalized with Hsc70. Data are expressed relatively to the ratio found in control mice arbitrarily set at 1. Aniline hydroxylase activity supported by CYP2E1 was measured in liver homogenates and results are expressed as nmol/min/mg protein. Data are mean±s.e.m. for 18–28 mice (18 mice in DDTC and DDTC+CCl4 groups and 28 mice in control and CCl4 groups). *Significantly different from control mice (P<0.05). #Significantly different from CCl4-treated mice (P<0.05).
