Figure 6
Effect of DDTC on CCl4-induced modifications of stress and defense-related proteins. Mice were treated or not for 3 and 24 h with 1.5 ml CCl4, with or without pretreatment with DDTC. Transcript levels of (a) HO-1, (c) Hsp70 and (e) MnSOD were measured by RT-qPCR. Expression of S6 was used to normalize mRNA levels. (b) HO-1, (d) Hsp70 and (f) MnSOD protein expression levels were assessed by western blotting and normalized with Hsc70. Data are expressed relatively to the ratio found in control mice arbitrarily set at 1 and are means±s.e.m. for 18 different mice in each group at the 3 h timepoint (three series with six mice per group) and for six mice per group at the 24 h timepoint. *Significantly different from control mice (P<0.05). #Significantly different from CCl4-treated mice (P<0.05).
