Figure 7
Prevention by DDTC of CCl4-induced mitochondrial dysfunction. Mice were treated or not for 3 h with 1.5 ml CCl4, with or without pretreatment with DDTC. MtDNA (a) depletion and (b) damage were assessed by RT-qPCR and long PCR, respectively. (c) A representative picture of an agarose gel with bands corresponding to the amplifications of the short (317 bp) and the long fragment (6365 bp) obtained by long PCR is shown. The activity of mitochondrial respiratory chain (d) complex I and (e) complex IV were measured by spectrophotometry. Data are mean±s.e.m. for 18–28 mice per group. *Significantly different from control mice (P<0.05). #Significantly different from CCl4-treated mice (P<0.05).
