Table 3 Effects of antioxidants on early (3 h) CCl4-induced modifications

From: Carbon tetrachloride-mediated lipid peroxidation induces early mitochondrial alterations in mouse liver

 

Control

CCl4

DHEA

DHEA+CCl4

TROLOX

TROLOX+CCl4

ALT (IU/l)

67.4±7.7

223.4±46.2a

89.5±22.3

151.5±22.0

99.3±20.9

314.8±41.7a

AST (IU/l)

129.7±24.3

473.3±91.4a

177.0±26.7

256.4±35.5

133.5±20.0

534.4±116.7a

TBARS (nmol/mg protein)

0.11±0.01

0.15±0.01a

0.10±0.01

0.09±0.01b

0.12±0.02

0.09±0.01b

Complex IV (nmol/min/mg protein)

20.6±2.1

2.0±0.7a

16.8±0.9

13.0±0.8b

28.0±6.0

18.6±5.4b

mtDNA/nDNA (fold-change)

1.00±0.11

0.78±0.07a

0.89±0.11

1.01±0.11b

1.03±0.09

0.85±0.09

Long/short mtDNA fragments (fold-change)

1.00±0.05

0.55±0.08a

0.94±0.06

1.01±0.07b

0.98±0.06

0.99±0.03b

CY2E1/S6 (fold-change)

1.00±0.09

0.42±0.04a

0.93±0.03

0.45±0.04a

0.78±0.07

0.40±0.03a

HO-1/S6 (fold-change)

1.0±0.2

18.0±3.6a

0.9±0.1

20.3±4.5a

1.0±0.1

30.4±3.5a

Hsp70/S6 (fold-change)

1.0±0.1

82.9±8.1a

1.5±0.1

114.8±33.6a

1.2±0.2

145.1±16.7a,b

MnSOD/S6 (fold-change)

1.00±0.05

0.81±0.03a

0.91±0.09

0.82±0.06

0.96±0.13

0.86±0.07

  1. Data are mean±s.e.m. for 12 mice per group.
  2. aSignificantly different from control mice (P<0.05).
  3. bSignificantly different from CCl4-treated mice (P<0.05).