Figure 1 | Laboratory Investigation

Figure 1

From: Gα12 activation in podocytes leads to cumulative changes in glomerular collagen expression, proteinuria and glomerulosclerosis

Figure 1

Endogenous Gα12 is expressed in normal mouse kidney. (a) Immunohistochemistry of normal mouse kidney demonstrates Gα12 expression in glomeruli. Sections were probed with rabbit anti-Gα12 and visualized with Vectastain. Two magnifications ( × 20 and × 40) are shown (panels A and B) Negative controls were performed by pre-incubating the antibody with excess blocking peptide (Gα12+Pep). Kidney sections using the blocked Gα12 antibody showed a significant reduction in staining (panels B and D). (b) Immunoelectron microscopy shows that Gα12 localizes to interdigitating foot processes (FP) and major processes (MP). Immunogold labeling and electron microscopy were performed as described in Materials and Methods. Magnification, × ∼100 000. Arrows denote gold particles. Glomerular basement membrane (GBM), FP, fenestrated endothelium (E) and larger major processes (MP) are labeled.

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