Figure 3
QLα12LacZ+/Cre+ mice develop of proteinuria with age. (a) Urine microalbumin/creatinine ratio in QLα12 mice is higher than in controls. Urine from control and QLα12 mice was monitored every 2 months using a BCA analyzer. Albumin/creatinine from individual control (○) and QLα12 (▪) mice are shown. Lines indicate median value (dashed, control; solid, QLα12). (b) Urine from QLα12 mice contains high levels of albumin. Urine from 12- and 16-month QLα12 (QL) and littermate control mice (c) was collected and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie blue staining. The arrow denotes ∼66 kDa, the size of excreted albumin. Note that the 12-month old mice had more concentrated urine (based on the nonspecific low-molecular-weight bands). (c) QLα12 mice are more susceptible to lipopolysaccharide (LPS)-induced injury. Control (n=17) and QLα12 (n=21) mice were injected with 10 μg/g body weight of LPS. Urine was collected 18 h post-injection and analyzed for urine microalbumin/creatinine ratio using a BCA analyzer. Statistical analysis was performed using two-way analysis of variance (ANOVA), followed by Bonferroni's post-hoc test (#P<0.001; *P<0.0001).
